Multiple sequence alignment of human a-TTP

This alignment was performed with the Multalin service of the Network Protein Sequence @nalysis, provided by Pole Bio-Informatique Lyonnaise


                       10        20        30        40        50        60
                        |         |         |         |         |         |
aTTP_32kDa     ------------------------------------------MAEARSQPSAGPQLNALP
CRAL_human     MSEGVGTFRMVPEEEQELRAQLEQLTTKDHGPVFGPCSQLPRHTLQKAKDELNEREETRE
TAP_45kDa      -------------------------------------------------------MSGRV
PTPASE_MEG2    ---------------------------------------------MEPATAP---RPDMA
Sec14p         -------------------------------------QQEKEFLESYPQNCPPDALPGTP
Consensus                                                                  
Prim.cons.     MSEGVGTFRMVPEEEQELRAQLEQLTTKDHGPVFGPC2Q22233E44PQ44P333LPGRP

                       70        80        90       100       110       120
                        |         |         |         |         |         |
aTTP_32kDa     DHSPLLQPGLAALRRRAREAGVPLAPL--PLTDSFLLRFLRARDFDLDLAWRLLKNYYKW
CRALBP_human   EAVRELQEMVQAQAASGEELAVAVAERVQEKDSGFFLRFIRARKFNVGRAYELLRGYVNF
TAP_45kDa      GDLSPRQKEALAKFRENVQD-V-LPALPNPDDYFL-LRWLRARSFDLQKSEAMLRKHVEF
PTPASE_MEG2    PELTPEEEQATKQFLEEINKWT-VQYNVSPLSWNVAVKFLMARKFDVLRAIELFHSYRET
Sec14p         GNLDSAQEKALAELRKLLED----AGFIERLDDSTLLRFLRARKFDVQLAKEMFENCEKW
Consensus        l   #e a a  r   #  v  a    pld    lrflrARkF#v  a e$l  y   
Prim.cons.     G5L5PLQE5ALAQFRE55ED3V22A5LV4PLDDSFLLRFLRARKFDVQ2A5ELLRNYV22

                      130       140       150       160       170       180
                        |         |         |         |         |         |
aTTP_32kDa     RAEC-------------PEISADLHPRSIIGLLKAGYHGVLRSRDPTGSKVLIYRIAHWD
CRALBP_human   RLQY-------------PELFDSLSPEAVRCTIEAGYPGVLSSRDKYGRVVMLFNIENWQ
TAP_45kDa      RKQKDIDNIIS---WQPPEVIQQYLSGGMCGYDLDGCPVWYDIIGPLDAKGLLFSASKQD
PTPASE_MEG2    RRKEGIVKLKP---HEEP-LRSEILSGKF---------TILNVRDPTGASIALFTARLHH
Sec14p         RKDYGTDTILQDFHYDEKPLIAKFYPQYYHKTDKDGRPVYFEELGAVNLHEMNKVTSEER
Consensus      R                pel     p         g p  l  rdp g    lf      
Prim.cons.     RKQYGID3I33DFH33EPELIA5LLPG554GTDK2GYP2VL5SRDPTGAKV2LF52S5WD

                      190       200       210       220       230       240
                        |         |         |         |         |         |
aTTP_32kDa     PKVFTAYDVFRVSLITSELIVQEVETQRNGIKAIFDLEGWQFSHAFQITPSVAKKIAAVL
CRALBP_human   SQEITFDEILQAYCFILEKLLENEETQINGFCIIENFKGFTMQQAASLRTSDLRKMVDML
TAP_45kDa      LLRTKMREC-ELLLQECAHQTTKLGRKVETITIIYDCEGLGLKHLWKPAVEAYGEFLCMF
PTPASE_MEG2    PHKSVQHVVLQALFYLLDRAVDSFETQRNGLVFIYDMCGSNYANF---ELDLGKKVLNLL
Sec14p         MLKNLVWEYESVVQYRLPACSRAAGHLVETSCTIMDLKGISISSAYS-VMSYVREASYIS
Consensus             e        l       etq #g   I #  G     a     s   k    l
Prim.cons.     PLK5T55EVLQ2LLY5LE55V555ETQ2NGICIIYDL2G555SHA4S355S552K5L5ML

                      250       260       270       280       290       300
                        |         |         |         |         |         |
aTTP_32kDa     TDSFPLKVRGIHLINEPVIFHAVFSMIKPFLTEKIKERIHMHGNNYKQSLLQHFP-DILP
CRALBP_human   QDSFPARFKAIHFIHQPWYFTTTYNVVKPFLKSKLLERVFVHGDDLSGFYQEIDE-NILP
TAP_45kDa      EENYPETLKRLFVVKAPKLFPVAYNLIKPFLSEDTRKKIMVLGANWKEVLLKHISPDQVP
PTPASE_MEG2    KGAFPARLKKVLIVGAPIWFRVPYSIISLLLKDKVRERIQILKTS---EVTQHLPRECLP
Sec14p         QNYYPERMGKFYIINAPFGFSTAFRLFKPFLDPVTVSKIFILGSSYQKELLKQIPAENLP
Consensus         %P r k    ! aP  F   %  ikpfL  k  er!  lg      ll h p # lP
Prim.cons.     QDSFP2RLKKIHIINAP55F52AY2LIKPFLKEKTRERIF2LG52YK4ELL2HIP32ILP

                      310       320       330       340       350       360
                        |         |         |         |         |         |
aTTP_32kDa     LEYGG--EEFSMEDICQEWTNFIMKSEDYLSSISESIQ----------------------
CRALBP_human   SDFGG--TLPKYDGKAVAEQLFGPQAQAENTAF---------------------------
TAP_45kDa      VEYGGTMTDP--DGNPKCKSKINYGGDIPRKYYVRDQVKQQYEHSVQISRGSSHQVEYEI
PTPASE_MEG2    ENLGGYVKIDLATWNFQFLPQVNGHPDPFDEIILFSLPPALDWDSVHVPGPHAMTIQELV
Sec14p         VKFGGKSEVDESKGGL-YLSDIGPWRDPKYIGPEGEAPE---------------------
Consensus         GG        g            #                                 
Prim.cons.     VE2GG3325244DGN5Q5LS522P55DP5555I44S4P322222SV22222222222222

                      370       380       390       400       410       420
                        |         |         |         |         |         |
aTTP_32kDa     ------------------------------------------------------------
CRALBP_human   ------------------------------------------------------------
TAP_45kDa      LFPGCVLRWQFMSDGADVG----FGIFLKTKMGERQRAGEMTEVLPNQRYNSHLVPEDG-
PTPASE_MEG2    DYVNARQKQGIYEEYEDIRRENPVGTFHCSMSPGNLEKNRYGDVPCLDQTRVKLTKRSGH
Sec14p         ------------------------------------------------------------
Consensus                                                                  
Prim.cons.     2222222222222222D22RENP2G2F2222222222222222V222222222L2222GH

                      430       440       450       460       470       480
                        |         |         |         |         |         |
aTTP_32kDa     ------------------------------------------------------------
CRALBP_human   ------------------------------------------------------------
TAP_45kDa      TLTCSDPGIYVLRFDNTYSFIHAKKVNFTVEVLLPDKASEEKMKQLGAGTPK--------
PTPASE_MEG2    TQTDYINASFMDGYKQKNAYIGTQGPLENTYRDFWLMVWEQKVLVIVMTTRFEEGGRRKC
Sec14p         ------------------------------------------------------------
Consensus                                                                  
Prim.cons.     T2T22222222222222222I222222222222222222E2K2222222T22EEGGRRKC

                      490       500       510       520       530       540
                        |         |         |         |         |         |
aTTP_32kDa     ------------------------------------------------------------
CRALBP_human   ------------------------------------------------------------
TAP_45kDa      ------------------------------------------------------------
PTPASE_MEG2    GQYWPLEKDSRIRFGFLTVTNLGVENMNHYKKTTLEIHNTEERQKRQVTHFQFLSWPDYG
Sec14p         ------------------------------------------------------------
Consensus                                                                  
Prim.cons.     GQYWPLEKDSRIRFGFLTVTNLGVENMNHYKKTTLEIHNTEERQKRQVTHFQFLSWPDYG

                      550       560       570       580       590       600
                        |         |         |         |         |         |
aTTP_32kDa     ------------------------------------------------------------
CRALBP_human   ------------------------------------------------------------
TAP_45kDa      ------------------------------------------------------------
PTPASE_MEG2    VPSSAASLIDFLRVVRNQQSLAVSNMGARSKGQCPEPPIVVHCSAGIGRTGTFCSLDICL
Sec14p         ------------------------------------------------------------
Consensus                                                                  
Prim.cons.     VPSSAASLIDFLRVVRNQQSLAVSNMGARSKGQCPEPPIVVHCSAGIGRTGTFCSLDICL

                      610       620       630       640       650       660
                        |         |         |         |         |         |
aTTP_32kDa     ------------------------------------------------------------
CRALBP_human   ------------------------------------------------------------
TAP_45kDa      ------------------------------------------------------------
PTPASE_MEG2    AQLEELGTLNVFQTVSRMRTQRAFSIQTPEQYYFCYKAILEFAEKEGMVSSGQNLLAVES
Sec14p         ------------------------------------------------------------
Consensus                                                                  
Prim.cons.     AQLEELGTLNVFQTVSRMRTQRAFSIQTPEQYYFCYKAILEFAEKEGMVSSGQNLLAVES

               
               
aTTP_32kDa     -
CRALBP_human   -
TAP_45kDa      -
PTPASE_MEG2    Q
Sec14p         -
Consensus                                                                  
Prim.cons.     Q                                                           


Alignment data :
Alignment length : 661
Residues conserved for 90 % or more (upper-case letters) : 13 is 1.97 %
Residues conserved for 50 % and less than 90 % (lower-case letters) : 62 is 9.38 %
Residues conserved less than 50 % (white space) : 574 is 86.84 %
IV conserved positions (!) : 2 is 0.30 %
LM conserved positions ($) : 1 is 0.15 %
FY conserved positions (%) : 2 is 0.30 %
NDQEBZ conserved positions (#): 7 is 1.06 %
Sequence 0001 : aTTP_32kDa ( 278 residues).
Sequence 0002 : CRALBP_human ( 317 residues).
Sequence 0003 : TAP_45kDa ( 403 residues).
Sequence 0004 : PTPASE_MEG2 ( 593 residues).
Sequence 0005 : Sec14p ( 296 residues).

MULTALIN options used :
c=blosum62
g=12
k1=90
k2=50
l=2
order=optA
s=abs